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why we use potassium dichromate in uv calibration

Description: Potassium Dichromate dissolved in Perchloric Acid (0. 001N) is a tried and true method of verifying for absorbance accuracy (photometric) as well as linearity in the UV range. Producing several characteristic peaks throughout the UV range, Potassium Dichromate enables checking at 235nm, 257nm, 313nm, 350nm and 430nm. FireflySci produces its own blend in house, derived from the NIST SRM 935a guidelines and permanently seals the contents in a fire-fused quartz cuvette. Potassium Dichromate is specifically required by many labs conforming to European Pharmacopoeia (EP) as well as Current Good Manufacturing Practices (cGMP).


Validation is frequently required to be performed every 3 months or after repairs. If periodic calibration is not required by your particular lab, we strongly recommend choosing our FUV-series solid state calibration standards, which NEVER need to be recalibrated due to material aging. With just one filter, you can check for photometric accuracy and stray light for ANY points in the range of 200-700nm. Capabilities: Included with Potassium Dichromate Liquid Calibration Standard: Please allow one week of lead time from the time of order. All of our calibration standards are proudly made in North America.
How can I use Potassium Dichromate to verify the performance of my spectrophotometer?


The use of Potassium Dichromate solvated in dilute Perchloric acid is an established and well recognised method for the validation of the absorbance scale and linearity of a spectrophotometer in the UV region. When prepared in 0. 001M Perchloric acid, potassium dichromate gives a spectral scan containing characteristic peaks at 257 nm and 350 nm, and troughs at 235 nm and 313 nm. Within the concen tration range 20-100 mg/l, if the absorbance scale of a narrow SBW ( 2 nm) spectro photometer is linear, the apparent absorbance of a series of concentrations will be a linear function of concentration.


Reference kits that are used for testing the UV region of a Spectrophotometer, are supplied with a calibration certificate that indicates the absorbance of the peaks and the troughs. These values are for a specific bandwidth, typically 2nm, and are therefore valid only for spectrophotometers operating at that bandwidth. values that are obtained from an instrument with a bandwidth 2nm would be different to those indicated at 2nm. Thus, 2. 8 rating from 11 votes Your feedback is important to us. Why not take a moment to let us know how helpful you found our answer by using the star-rating.

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