why do we destarch plants in use for experiments
During photosynthesis, a plant produces glucose molecules. The glucose that is not used immediately by the plant is stored in the chloroplast and cytoplasm as starch. This starch can later be hydrolyzed back into glucose when needed. Placing a plant in dark conditions prevents photosynthesis form taking place and forces the plant to use its stored starch. In order to determine if a plant has been properly destarched, it must be chemically tested.
The most common way to do so involves taking a leaf from the destarched plant, boiling it in water, then boiling the leaf in ethanol in a hot water bath. Following this, a simple iodine test reveals if starch is still present in the leaf. Carbon dioxide deprivation can also be used to destarch a plant. This involves placing the plant in an enclosure, such as a large jar and sealing it shut. A sodium hydroxide solution or granular soda lime can be placed in the enclosure along with the plant to accelerate the process.
The purpose of conducting experiment of Photosynthesis is to understand what are the factors that influence photosynthesis, Rate of photosynthesis in different plants and the pathway how the photosynthesis occur i. e producing glucose using energy from sunlight, carbon dioxide and water.
The result for the experiments will be obtained from the amount of glucose produced.
During photosynthesis, a plant produces glucose molecules. The glucose that is not used immediately by the plant is stored in the chloroplast and cytoplasm as starch. This starch can later be hydrolyzed back into glucose when needed. If starch is already present at the beginning of the experiment the results will not be accurate. Hence, it is important to start off with a plant without starch. This is accomplished by de-starching
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